2006 - 75 (5)

Tijdens de eerste twee weken van maart 2004 werden er 80 zwarte kraaien (Corvus corone) dood aangetroffen in eenpark in Brussel (België). Bij de lijkschouwing werden splenomegalie, hemorragische enteritis met duodenum letstels encerebrale of meningeale bloedingen waargenomen die deden denken aan West Nile virusinfectie. Met realtimePCR (real -time omgekeerde transcriptie polymerasekettingreactie) werd geen amplificatie van de West Nile virus specifieke3’UTR RNA-sequentie waargenomen terwijl ook de virusisolatie van gehomogeniseerde hersenen negatief was. Toxi -cologisch onderzoek toonde geen toxinen aan. Transmissie-elektronenmicroscopie toonde intracytoplasmatische, reo -virusachtige partikels aan in ultradunne secties van de milt en het duodenum. De vermeerdering van dit virus in culturenvan embryonaire hepatocyten van de kip werd bewezen op basis van hun cytopathogeen effect en hun aanwezigheid ingeïnfecteerde cellen en hun supernatans.

A case of Ebstein’s anomaly leading to right-sided heart failure in a one-year-old female Golden Retriever is reported. Clinical examination and investigations such as blood analysis, ECG, X-Ray, echocardiography and conservative therapy are described. Finally, the post mortem examination of the heart clearly showed the distal displacement of the malformed tricuspid valve leaflets, chordae tendinae and papillary muscles in the right ventricle, which is characteristic of Ebstein’s anomaly.

A two-month-old male non-diabetic Holstein Friesian calf with numerous decubital ulcers due to arthrogryposis and a history of inability to stand since birth was diagnosed with emphysematous cystitis. The diagnosis was based on the necropsy and the histopathological findings. No glucosuria was detected and Escherichia coli was isolated from the urine and blood. The contamination of the skin ulcers with E. coli and septicemia was probably the main underlying mechanism involved in the pathogenesis. The urinary bladder mucosa was affected with blebs of emphysema and petechiaes. Histopathologically, hemorrhage, emphysema and a few neutrophil leukocyte infiltrations were observed in the mucosa of the urinary bladder. Although emphysematous cystitis is commonly associated with glucosuria, especially in dogs, this report indicates that it can equally occur as a complication of septicemia in calves.