This case report describes the surgical procedure for prepubic urethrostomy in the female dog, documented in a case in which it was used after resection of vaginal neoplasia. Prepubic urethrostomy is indicated as a salvage procedure in cases where there is not sufficient urethral length left to perform a perineal urethrostomy. This loss of urethral length can be due to extensive resection for oncologic surgery or to extensive tissue trauma. The main complication that can occur in this type of urethrostomy is urinary incontinence.