Urinalysis plays an important role in diagnosing several diseases and as part of routine healthchecks of senior and geriatric patients. There is no solid data regarding the role of experience in themicroscopical examination of urine sediment of small animals. The purpose of this study was to investigatethe role of experience when examining urine sediment. In total, sediment of 27 urine samples(of 13 dogs and 14 cats) were microscopically examined independently by one experienced observer(the expert) and two unexperienced final-year, veterinary students. For most sediments, there was aweak agreement (low kappa value: 0-0.4) between the expert and the students, with the exception of amoderate agreement for struvite crystals (kappa value: 0.47). The agreement between the students wasalso weak, but slightly higher than the agreement between the expert and the students. In this study, itis shown that experience plays an important role in the correct examination of urine sediment.