Feline immunodeficiency virus (FIV) and feline leucosis virus (FeLV) are two pathogens in catsthat may have severe consequences on the general health of the infected animal, especially in the longterm. The stray cat population is a reservoir for maintaining the infection in this group but is also responsiblefor spreading the infection to housecats.For many years, the City of Ghent has been pursuing a stray cat control policy according to thetrap-neuter-return principle that entails euthanasia of stray cats affected by FIV or FeLV. To study theinfluence of the eradication plan on the prevalence of FIV and FeLV in the stray cat population, dataobtained in 2009 were compared to data of 2017. A significant drop in the infection rate was observedfor FeLV (from 9.9% to 0.7%). For FIV, the prevalence remained similar (from 9.1% to 10.3%). A shiftin the number of stray cats caught as well as in the percentage of positive cases was observed fromdowntown to the boundaries of the Ghent area. Various factors may have influenced these preliminaryfindings. Further research should elucidate factors responsible for the evolution of the FIV and FeLVprevalence in stray cats in the Ghent area.