At present, only 5% of pedigree dogs is being used for breeding. To increase the number ofbreeding dogs, one solution could be to start a canine semen bank based on the principle of semendonation, like in humans. Many dog owners have no desire to become dog breeders but are willingto preserve the genetic material of their dog, if offered this possibility. However, not all canineejaculates are suitable for cryopreservation as the initial quality may differ and the resistance ofsperm cells to survive the freezing procedure is highly variable. In order to freeze the semen ofas many male dogs as possible, it is important to optimize and individualize the cryopreservationprotocol per ejaculate. Practically, frozen semen can be stored in the CanIfreeze-semen bank orin veterinary practices adjacent to the owner of the bitch and can be used for insemination at alater time.