Barium sulphate is an insoluble contrast medium that is commonly used for radiographyof the digestive tract. During examination, aspiration of barium might occur and cause severecomplications in both humans and animals. In this report, a case of barium aspiration duringgastrointestinal examination of a four-year-old, spayed, female Persian cat is described.Radiographic and pathological findings confirmed the diagnosis of barium aspiration.