A young male domestic Shorthair was presented with weakness and anorexia of two days’ duration.Physical examination showed pale mucous membranes, caused by severe, regenerative,Coombs’ positive, hemolytic anemia. A blood smear revealed epicellular organisms compatible withMycoplasma spp. Real-time polymerase chain reaction (RT-PCR) on EDTA blood identified these organismsas Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’. Despite thelack of clearance of the organism from the blood, the cat responded well to antibiotic treatment withdoxycycline, together with immunosuppressive doses of corticosteroids.