The transport and artificial insemination of chilled (4°C) and cryopreserved (-196°C) dog semenhave gained increasing interest worldwide and have become very popular among dog breeders.Whereas cryopreservation of dog sperm is a complicated and time consuming procedure, which isalmost exclusively performed at universities, the chilling of dog semen can be handled by veterinariansin their private practices, provided that the basic knowledge of chilling and diluting semen isacquired. Immediately after sperm collection, the quality of the fresh sample is evaluated and recordedbefore diluting in an appropriate extender. Subsequently, the diluted semen is gradually chilledto 4°C. It can be stored at 4°C for several days or transported in a thermos flask, a styrofoambox or a Minitübe neopore box. Cryopreserved dog sperm is mostly transported in a dry-shippercontainer. The rules and legislation for the shipment of chilled and frozen dog semen are rathercomplicated. They differ between almost every country and may change over time. To comply withall the administrative procedures, it is necessary to plan the transport of semen well in advance.