A 6-year-old, male striped skunk (Mephitis mephitis) weighing 5.9 kg was anesthetized for diagnosticimaging procedures and subsequently for a laminectomy at level L6/S1 as treatment of lumbosacral stenosis.On both occasions, anesthesia was induced by a face mask using 5% isoflurane in oxygen. After endotrachealintubation, anesthesia was maintained with isoflurane in oxygen. When necessary, intermittent positivepressure ventilation was instituted.For the surgical intervention, analgesia was provided with pre-operative carprofen, perioperativeintravenous infusion of fentanyl and postoperative buprenorphine. The constant rate infusion of fentanylprovided a sufficient level of analgesia and reduced the amount of isoflurane needed. Hypoxemia occurred atthe end of surgery and was successfully treated using a vital capacity manoeuvre. The recovery from bothanesthetic procedures was smooth and uneventful and the surgical intervention was successful. Three weeksafter surgery the skunk was able to move the pelvic limbs voluntarily.