This paper describes the birth of the first intracytoplasmic sperm injection (ICSI) foal in the Benelux.Oocytes were aspirated from ovaries from slaughtered mares. After in vitro maturation, the oocytes werefertilized by ICSI and cultured in vitro for 9 days. Two embryos reached the blastocyst stage and they weretransferred to the uterus of a synchronized mare. Six days later a single embryonic vesicle was diagnosed byultrasound. After a normal pregnancy a healthy foal was born on the 27th of October 2009. Parentage testingvia microsatellite genotyping confirmed that the foal originated from the transferred embryo.