A new test for genotyping codons 136-154-171 of the ovine prion protein (PRNP) gene via the detection ofmutations of the PrP gene through reverse hybridization has been evaluated on non-coagulated blood andbrain tissue. A total of 100 sheep blood samples and 28 brain tissue samples were tested. These samples werealso analyzed with denaturating gradient gel electrophoresis and real-time polymerase chain reaction toconfirm the results obtained via the present test. The results obtained via these three tests correspondedperfectly. The paper briefly describes the use of the new test.