A set of two biochemical analysers which is commercially available for use in animals was evaluated for the determination of alanine aminotransferase, albumin, alkaline, phosphatase, amylase, aspartate aminotransferase, calcium, cholesterol, creatine kinase, creatinine, gamma-glutamyltranspeptidase, globulin, glucose, inorganic phosphate, lactate dehydrogenase, lipase, total bilirubin, total protein, triglycerides, urea nitrogen, uric acid, sodium, potassium and chloride concentrations in pigeon plasma. The results indicated that the analysers are reliable for use in this bird species. Reference values for all parameters were established through examination of healthy pigeons.