84 (2) pg 80-87

Title: 
Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study
Author(s): 
N. VAN DER VEKENS, A. DECLOEDT., D. DE CLERCQ, S. VEN, G. VAN LOON
Abstract: 

Brain natriuretic peptide (BNP) is used in human medicine for the diagnosis of congestiveheart failure. Since BNP is species-specific and no equine assay is available, BNP has never beendetermined in horses. Because there is more than 90% homology between porcine and equineBNP, a porcine BNP enzyme-linked immunoassay (ELISA) was used in the present study tomeasure BNP in plasma of healthy horses (group 1; n=20), horses with cardiac disease without(group 2a; n=8) and with atrial dilatation (n=8), ventricular dilatation (n=1) or both (n=1) (group2b; n=10). Samples were stored at -20°C and -80°C to study the influence of storage temperature.No significant differences were found between the BNP concentrations of group 1 (77.79; 37.20-513.36 pg/mL), group 2a (52.02; 24.69-268.37 pg/mL) or 2b (94.73; 42.88-470.66 pg/mL). Samplesstored at -80°C showed significantly (72.19, 24.69-513.36 pg/mL; P=0.001) higher concentrationsthan samples stored at -20°C (47.35, 24.69-430.60 pg/mL). In this pilot study, it is suggested thatthe porcine BNP assay does not allow accurate detection of equine BNP. An equine specific BNPassay should be developed to study BNP concentrations in horses.   

Full text: 
pp 080-087
Original article(s)