80 (2) pp 129-136

Title: 
Purification and expansion of stem cells from equine peripheral blood, with clinical applications
Author(s): 
J.H. SPAAS, A. GAMBACURTA, M. POLETTINI, S. BROECKX, F. VAN HOECK, C. DE SCHAUWER, G.R. VAN DE WALLE, A. VAN SOOM
Abstract: 

Equine peripheral blood (ePB) can be used as a source of stem cells (SCs) in horses, both for research andfor practical purposes. A relatively low volume of ePB is sufficient for the purification and expansion of theSCs. The identification of the SCs is performed by demonstrating the presence (CD34, CD90, CD105 andCD117) or absence (CD14) of specific markers on the cell surface by means of fluorescent staining, followedby Fluorescence Activated Cell Sorting (FACS) for sorting out the desired population of SCs. The entire processof SC isolation and enrichment from ePB typically takes three days, after which the enriched SC sample canbe sent back to the patient for clinical application. The two most common clinical applications of SCs from ePBwill be demonstrated with two field cases. The first case presents a lesion of the body of the suspensory ligamentin a 13-year-old warmblood mare and the second case describes a bacterial ulcerative keratitis in a 20-yearoldwarmblood gelding.

Full text: 
pp 129-136
Case report(s)